RECOMBINANT DNA MANIFESTATION VECTOR AND DNA COMPOUND ENCODING ISOPENICILLIN-N SYNTHETASE OF PENICILLIUM CRYSOGENUM

Japanese Patent JPS62130691

A

The present invention comprises novel DNA com­pounds that encode isopenicillin N synthetase. The invention also comprises methods, transformants, and polypeptides related to the novel DNA compounds. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcription and translation activating sequence, was isolated from Penicilliumchrysogenum. The isopenicillin N synthetase-encoding DNA can be used to construct novel E. coli expression vectors that drive expression of isopenicillin N syn­thetase in E. coli. The intact P. chrysogenum iso­penicillin N synthetase-encoding DNA and associated transcription and translation activating sequence can also be used to construct expression vectors that drive expression of the isopenicillin N synthetase in P. chrysogenum and Cephalosporiumacremonium. The transcription and translation activating sequence can be fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto expression vectors that function in P. chrysogenum and C. acremonium. The transcription termination and mRNA polyadenylation signals of the P. chrysogenum isopenicillin N synthetase can be used to increase ultimate expression of a product encoded on a recombinant DNA vector.

CARR LUCINDA GAYLE (US)
INGOLIA THOMAS DOMINICK (US)
QUEENER STEPHEN WYATT (US)
SKATRUD PAUL LUTHER (US)

JP28161386A

June 12, 1987

November 25, 1986

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LILLY CO ELI

C12N1/14; C12N1/15; C12N1/20; C12N1/21; C12N9/00; C12N15/00; C12N15/52; C12N15/69; C12N15/09; C12N15/70; C12N15/80; C12N15/90; C12P37/00; C12R1/19; C12R1/75; C12R1/82; (IPC1-7): C12N1/14; C12N1/20; C12N9/00; C12N15/00; C12P37/00; C12R1/19; C12R1/75; C12R1/82

Aoyama Aoi

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