Title:
Recombination humans' DNaseI manufacturing method
Document Type and Number:
Japanese Patent JP6071018
Kind Code:
B2
Abstract:
Disclosed is a method for production of recombinant human DNase I which is of such high purity as may be directly used as a medical drug. The method comprises the steps of; culturing recombinant DNase I-producing mammalian cells, subjecting a culture supernatant to an anion-exchange column chromatography, subjecting to a column chromatography employing as solid phase a material having affinity for phosphate group, subjecting to a cation-exchange column chromatography, and subjecting to a dye affinity column chromatography.
Inventors:
Koshimura Yuri
Matev Milos Love
Hiroyuki Sonoda
Matev Milos Love
Hiroyuki Sonoda
Application Number:
JP2015205158A
Publication Date:
February 01, 2017
Filing Date:
October 19, 2015
Export Citation:
Assignee:
jcr pharma corporation
International Classes:
C12N9/16; C12N15/09
Domestic Patent References:
| JP2005524092A | ||||
| JP2001157580A | ||||
| JP2014508506A | ||||
| JP9512715A |
Foreign References:
| WO2012063799A1 |
Other References:
J. Biochem., 1984, Vol. 95, pp. 1399-1406
Attorney, Agent or Firm:
Yoshiki Yamamoto
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