Title:
組み換えインスリン様成長因子IGF-I様ポリペプチドの再折りたたみ
Document Type and Number:
Japanese Patent JP3648242
Kind Code:
B2
Abstract:
A composition is provided comprising about 0.1 to 15 mg/mL of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3 M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9 M of a chaotropic agent, and about 0.01 to 15 mu M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.
Inventors:
Builders, Stuart
Heart, roger
Leicester, Philip
Raf snider, david
Heart, roger
Leicester, Philip
Raf snider, david
Application Number:
JP50762695A
Publication Date:
May 18, 2005
Filing Date:
August 15, 1994
Export Citation:
Assignee:
Genentech, Inc.
International Classes:
C12P21/02; C07K1/113; C07K1/14; C07K14/48; C07K14/61; C07K14/65; C12N15/18; A61K38/00; C12R1/19; (IPC1-7): C07K14/65; C07K14/48; C07K14/61; C12P21/02
Domestic Patent References:
| JP1131195A |
Foreign References:
| WO1993011240A1 | ||||
| WO1991000344A1 |
Attorney, Agent or Firm:
Sonoda Yoshitaka
Kobayashi Yoshinori
Kobayashi Yoshinori