To provide a regulator for a culture vessel, capable of suitably operating the culture vessel by calculating the state of an arbitrary point in the culture vessel by the numerical value simulation of flow; and to provide a culture device.
The regulator has an input means, and a calculating means. The input means inputs measured data obtained from a measuring means for measuring a nutrient composition, an oxygen concentration, a carbon dioxide concentration and a biomass concentration in a medium. The calculating means calculates the consumption rate of the nutrient composition, the consumption rate of the oxygen and the discharging rate of the carbon dioxide per unit amount of the biomass from the measured data input by the input means, calculates a mass transfer volume coefficient kLa calculated from a turbulent energy k and a turbulent energy dissipation calculated from a transport equation, and a diffusion coefficient D, and calculates the nutrient composition concentration, the dissolved oxygen concentration and the dissolved carbon dioxide concentration in an arbitrary region in the culture vessel by using an algorithm for numerically integrating a differential equation explaining temporal change of the medium components from the calculated consumption rate of the nutrient composition, the consumption rate of the oxygen, the consumption rate of the carbon dioxide, and the mass transfer volume coefficient kLa. The culture device is also provided.
JPN7011000230, 海野肇、他著， 「バイオプロセス工学 計測と制御」， 株式会社講談社サイエンティフィク， （１９９７）， ｐ．９０−１３９
JPN6011003272, 醗酵工学， （１９８７）， ６５， ［１］， ｐ．５９−６９
JPN7011000231, Ｔｒａｎｓ， ＩＣｈｅｍＥ， （１９９６）， ７４， ［Ｐｔ．Ａ］， ｐ．４９２−４９８
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