Title:
REMOVAL OF HIGH-MOLECULAR-WEIGHT AGGREGATE USING HYDROXYAPATITE CHROMATOGRAPHY
Document Type and Number:
Japanese Patent JP2012111769
Kind Code:
A
Abstract:
To provide a method of purifying an antibody monomer from a preparation that contains a high-molecular-weight aggregate.
The present invention relates to the method of purifying the antibody monomer from the preparation that contains the high-molecular-weight aggregate, including: a step of applying the antibody preparation to hydroxyapatite chromatography; and a step of further applying the antibody preparation to protein-A affinity chromatography and ion exchange chromatography.
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Inventors:
SUN SHUJUN
GALLO CHRISTOPHER
KELLEY BRIAN
GALLO CHRISTOPHER
KELLEY BRIAN
Application Number:
JP2012013648A
Publication Date:
June 14, 2012
Filing Date:
January 26, 2012
Export Citation:
Assignee:
WYETH LLC
International Classes:
C07K1/16; B01D15/26; B01D15/38; B01J20/282; C07K16/00; C07K16/06; C07K16/22; A61K39/395; B01D15/36; C12P21/08
Domestic Patent References:
| JPH03291300A | 1991-12-20 | |||
| JPH03291300A | 1991-12-20 |
Other References:
JPN5006017844; Ｂｉｏｔｅｃｈｎｏｌ． Ｂｉｏｅｎｇ． Ｖｏｌ． ７３， Ｎｏ． ６, 2001, ｐｐ．５２２-５２９
JPN5006017845; Ｂｉｏｓｅｐａｒａｔｉｏｎ Ｖｏｌ． ９， Ｎｏ． ６, 2000, ｐｐ．３５９-３６８
JPN5006017846; Ｊ． Ｃｈｒｏｍａｔｏｇｒ． Ｖｏｌ． ５９９, 1992, ｐｐ．１３-２０
JPN5006017847; Ｂｉｏｌ． Ｃｈｅｍ． Ｈｏｐｐｅ-Ｓｅｙｌｅｒ Ｖｏｌ． ３７２， Ｎｏ． ３, 1991, ｐｐ．１４９-１５６
JPN5006017848; ＡＭＥＲＩＣＡＮ ＢＩＯＴＥＣＨＮＯＬＯＧＹ ＬＡＢＯＲＡＴＯＲＹ Ｖｏｌ． ３， Ｎｏ． ５, 1985, ｐｐ．２２-３０
JPN5006017849; ＪＯＵＲＮＡＬ ＯＦ ＣＨＲＯＭＡＴＯＧＲＡＰＨＹ Ｖｏｌ． ４７６, 19890804, ｐｐ．２６９-２９０
JPN6010038229; Ｊ． Ｉｍｍｕｎｏｌ． Ｍｅｔｈｏｄｓ Ｖｏｌ． ２３５, 2000, ｐｐ．６１-６９
JPN5006017844; Ｂｉｏｔｅｃｈｎｏｌ． Ｂｉｏｅｎｇ． Ｖｏｌ． ７３， Ｎｏ． ６, 2001, ｐｐ．５２２-５２９
JPN5006017845; Ｂｉｏｓｅｐａｒａｔｉｏｎ Ｖｏｌ． ９， Ｎｏ． ６, 2000, ｐｐ．３５９-３６８
JPN5006017846; Ｊ． Ｃｈｒｏｍａｔｏｇｒ． Ｖｏｌ． ５９９, 1992, ｐｐ．１３-２０
JPN5006017847; Ｂｉｏｌ． Ｃｈｅｍ． Ｈｏｐｐｅ-Ｓｅｙｌｅｒ Ｖｏｌ． ３７２， Ｎｏ． ３, 1991, ｐｐ．１４９-１５６
JPN5006017848; ＡＭＥＲＩＣＡＮ ＢＩＯＴＥＣＨＮＯＬＯＧＹ ＬＡＢＯＲＡＴＯＲＹ Ｖｏｌ． ３， Ｎｏ． ５, 1985, ｐｐ．２２-３０
JPN5006017849; ＪＯＵＲＮＡＬ ＯＦ ＣＨＲＯＭＡＴＯＧＲＡＰＨＹ Ｖｏｌ． ４７６, 19890804, ｐｐ．２６９-２９０
JPN6010038229; Ｊ． Ｉｍｍｕｎｏｌ． Ｍｅｔｈｏｄｓ Ｖｏｌ． ２３５, 2000, ｐｐ．６１-６９
JPN5006017845; Ｂｉｏｓｅｐａｒａｔｉｏｎ Ｖｏｌ． ９， Ｎｏ． ６, 2000, ｐｐ．３５９-３６８
JPN5006017846; Ｊ． Ｃｈｒｏｍａｔｏｇｒ． Ｖｏｌ． ５９９, 1992, ｐｐ．１３-２０
JPN5006017847; Ｂｉｏｌ． Ｃｈｅｍ． Ｈｏｐｐｅ-Ｓｅｙｌｅｒ Ｖｏｌ． ３７２， Ｎｏ． ３, 1991, ｐｐ．１４９-１５６
JPN5006017848; ＡＭＥＲＩＣＡＮ ＢＩＯＴＥＣＨＮＯＬＯＧＹ ＬＡＢＯＲＡＴＯＲＹ Ｖｏｌ． ３， Ｎｏ． ５, 1985, ｐｐ．２２-３０
JPN5006017849; ＪＯＵＲＮＡＬ ＯＦ ＣＨＲＯＭＡＴＯＧＲＡＰＨＹ Ｖｏｌ． ４７６, 19890804, ｐｐ．２６９-２９０
JPN6010038229; Ｊ． Ｉｍｍｕｎｏｌ． Ｍｅｔｈｏｄｓ Ｖｏｌ． ２３５, 2000, ｐｐ．６１-６９
JPN5006017844; Ｂｉｏｔｅｃｈｎｏｌ． Ｂｉｏｅｎｇ． Ｖｏｌ． ７３， Ｎｏ． ６, 2001, ｐｐ．５２２-５２９
JPN5006017845; Ｂｉｏｓｅｐａｒａｔｉｏｎ Ｖｏｌ． ９， Ｎｏ． ６, 2000, ｐｐ．３５９-３６８
JPN5006017846; Ｊ． Ｃｈｒｏｍａｔｏｇｒ． Ｖｏｌ． ５９９, 1992, ｐｐ．１３-２０
JPN5006017847; Ｂｉｏｌ． Ｃｈｅｍ． Ｈｏｐｐｅ-Ｓｅｙｌｅｒ Ｖｏｌ． ３７２， Ｎｏ． ３, 1991, ｐｐ．１４９-１５６
JPN5006017848; ＡＭＥＲＩＣＡＮ ＢＩＯＴＥＣＨＮＯＬＯＧＹ ＬＡＢＯＲＡＴＯＲＹ Ｖｏｌ． ３， Ｎｏ． ５, 1985, ｐｐ．２２-３０
JPN5006017849; ＪＯＵＲＮＡＬ ＯＦ ＣＨＲＯＭＡＴＯＧＲＡＰＨＹ Ｖｏｌ． ４７６, 19890804, ｐｐ．２６９-２９０
JPN6010038229; Ｊ． Ｉｍｍｕｎｏｌ． Ｍｅｔｈｏｄｓ Ｖｏｌ． ２３５, 2000, ｐｐ．６１-６９
Attorney, Agent or Firm:
Yoshinobu Murofushi
High Hoji Koji
Junko Miyazawa
Noh four
Maki Sato
Tatsuta Miyuki
High Hoji Koji
Junko Miyazawa
Noh four
Maki Sato
Tatsuta Miyuki