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Title:
ROLLING CIRCLE AMPLIFICATION OF CIRCULAR GENOME
Document Type and Number:
Japanese Patent JP2008161182
Kind Code:
A
Abstract:

To provide a method for exponential amplification of circular genomes for amplification of whole genomes.

The method of a rolling circle amplification of the circular genomes which involves strand replacement replication by primers, allows the exponential differential amplification of the circular genomes. In genomic nucleic acid samples containing both a circular genome of interest and non-target nucleic acids, such as non-target genomes, the disclosed methods and compositions can result in many-fold differential amplification of the circular genome of interest over non-target nucleic acids. The selection of a set of primers complementary to a circular genome of interest can result in much greater amplification of the circular genome of interest relative to non-target nucleic acids present. Such differential amplification of circular genomes is very useful for obtaining useful amounts of genomes of interest from a mixed nucleic acid sample.


Inventors:
KORFHAGE CHRISTIAN
LOEFFERT DIRK
Application Number:
JP2007276942A
Publication Date:
July 17, 2008
Filing Date:
October 24, 2007
Export Citation:
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Assignee:
QIAGEN GMBH
International Classes:
C12N15/09; C12Q1/68
Domestic Patent References:
JP2004512823A2004-04-30
JP2006512094A2006-04-13
Other References:
JPN6012054254; Journal of Virology Vol.78, No.10, 2004, p.4993-4998
Attorney, Agent or Firm:
Patent Service Corporation Patent Office Sykes