To provide a method for exponential amplification of circular genomes for amplification of whole genomes.
The method of a rolling circle amplification of the circular genomes which involves strand replacement replication by primers, allows the exponential differential amplification of the circular genomes. In genomic nucleic acid samples containing both a circular genome of interest and non-target nucleic acids, such as non-target genomes, the disclosed methods and compositions can result in many-fold differential amplification of the circular genome of interest over non-target nucleic acids. The selection of a set of primers complementary to a circular genome of interest can result in much greater amplification of the circular genome of interest relative to non-target nucleic acids present. Such differential amplification of circular genomes is very useful for obtaining useful amounts of genomes of interest from a mixed nucleic acid sample.
LOEFFERT DIRK
JP2004512823A | 2004-04-30 | |||
JP2006512094A | 2006-04-13 |
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