Title:
糖化アミンを測定するための試料の前処理方法および糖化アミンの測定方法
Document Type and Number:
Japanese Patent JP4590017
Kind Code:
B2
Abstract:
The present invention provides a method of measuring an amount of a glycated protein as an analyte in a sample, wherein the method comprises a pre-treatment step comprising causing a fructosyl amino acid oxidase (FAOD) to degrade and remove a glycated amino acid that can be present in the sample and which does not derive from the glycated protein; a degradation step with a protease to degrade the glycated protein to give a glycated peptide and/or a glycated amino acid; and a measurement step which comprises causing the FAOD to act on the glycated peptide and/or glycated amino acid to cause a redox reaction, which is measured to determine the amount of glycated protein.
Inventors:
Yonehara Satoshi
Komori Seedling
Komori Seedling
Application Number:
JP2010034254A
Publication Date:
December 01, 2010
Filing Date:
February 19, 2010
Export Citation:
Assignee:
Arkray Co., Ltd.
International Classes:
C12Q1/26; C12Q1/37; G01N33/48; G01N33/68; G01N33/72
Domestic Patent References:
| JP2001095598A | ||||
| JP2000333696A |
Attorney, Agent or Firm:
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Matsuda Nanae
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Matsuda Nanae