Title:
フラビウイルス持続感染細胞株による抗フラビウイルス感染化合物のスクリーニング方法
Document Type and Number:
Japanese Patent JP5290497
Kind Code:
B2
Abstract:
This invention provides a method for screening compounds against Flavivirus. More specifically, this invention provides a method for screening compounds against Flaviviruses by using a persistent virus-infected cell system, including (a) establishment of persistent virus-infected cell lines, (b) preparation of monoclonal antibody by using said persistent virus-infected cell lines, (c) incubation of tested compounds with said persistent virus-infected cell lines, (d) determination of the inhibition effect of tested compounds or vaccines on Flavivirus by sandwich ELISA using said monoclonal antibody.
Inventors:
Chen Ritsumitsu
Application Number:
JP2005373225A
Publication Date:
September 18, 2013
Filing Date:
December 26, 2005
Export Citation:
Assignee:
Bukkyo Jiju General Hospital
International Classes:
C12Q1/02; C12N5/07; C12N5/078; C12Q1/70; G01N33/15; G01N33/50; A61K45/00; A61P31/20; C12N5/071; C12N7/08
Domestic Patent References:
| JP2002356442A | ||||
| JP622799A | ||||
| JP200398170A | ||||
| JP2003510093A |
Other References:
Virology,1996,Vol.217,No.1,p.220-229
Journal of Virology,1998,Vol.72,No.12,p.9844-9854
Archives virology,1990,Vol.110,No.1-2,p.91-101
Journal of Virology,1998,Vol.72,No.12,p.9844-9854
Archives virology,1990,Vol.110,No.1-2,p.91-101
Attorney, Agent or Firm:
Yasuhiko Murayama
Masatake Shiga
Takashi Watanabe
Shinya Mitsuhiro
Masatake Shiga
Takashi Watanabe
Shinya Mitsuhiro