Title:
SELECTION OF EXTRANEOUS DNA FRAGMENT RECOMBINANT AND VECTOR FOR EXTRANEOUS GENE CLONING
Document Type and Number:
Japanese Patent JPH04365485
Kind Code:
A
Abstract:
PURPOSE: To obtain a novel vector used as the label for selecting only a transformer holding a recombinant into which an extraneous DNA fragment is integrated from among colonies of the transformer.
CONSTITUTION: A vector, e.g. plasmid pERISH1/JM109 (FERM P-11644), having a region of rcsB gene which is a regulatory gene of Escherichia coli polysaccharide synthesis gene (cps) and having a base sequence at cut position by a prescribed restriction enzyme (pstI, KpnI, etc.) in the rcsB gene region. The rcsB gene region is integrated into a plasmid pACYC184 to provide the objective plasmid.
Inventors:
ARAKAWA YOSHICHIKA
WACHIYAROTAYAKUN ROCHIYAPOON
HORII TOSHINOBU
OTA MICHIO
KATO NOBUO
WACHIYAROTAYAKUN ROCHIYAPOON
HORII TOSHINOBU
OTA MICHIO
KATO NOBUO
Application Number:
JP18695691A
Publication Date:
December 17, 1992
Filing Date:
July 02, 1991
Export Citation:
Assignee:
ARAKAWA YOSHICHIKA
International Classes:
C12N1/21; C12N15/09; C12N15/65; C12N15/70; C12R1/19; (IPC1-7): C12N1/21; C12N15/65; C12N15/70
Attorney, Agent or Firm:
Masatoshi Sato (1 person outside)