Title:
自己消光性蛍光プローブと方法
Document Type and Number:
Japanese Patent JP4131749
Kind Code:
B2
Abstract:
There is provided: a method for detecting a target polynucleotide in a sample comprising: contacting said sample of nucleic acids with an oligonucleotide probe under conditions favorable for hybridization; and monitoring the fluorescence of said reporter molecule, an increase in the fluorescence intensity of said reporter molecule indicating the presence of said target polynucleotide; wherein said oligonucleotide probe is an oligonucleotide probe comprising: an oligonucleotide probe which does not hybridize with itself to form a hairpin structure; a fluorescent reporter molecule attached to said oligonucleotide sequence; and a quencher molecule capable of quenching the fluorescence of said reporter molecule attached to said oligonucleotide sequence; said oligonucleotide sequence capable of existing in at least one single-stranded conformation when unhybridized where said quencher molecule is a fluorescent molecule capable of quenching the fluorescence of said reporter molecule; said oligonucleotide sequence also capable of existing in at least one conformation when hybridized to a target polynucleotide where the fluorescence of said reporter molecule is unquenched; and wherein the fluorescence intensity of said reporter molecule at its emission maximum when said oligonucleotide sequence is hybridized to said target polynucleotide is greater than the fluorescence intensity of said quencher molecule at its emission maximum when said oligonucleotide sequence is not hybridized to said target polynucleotide.
More Like This:
| WO/2003/095667 | AUTOIMMUNE CONDITIONS AND NADPH OXIDASE DEFECTS |
| WO/1992/015679 | IMPROVED EPITODE DISPLAYING PHAGE |
| JP2008000043 | ADP RIBOSYLATION PROTEIN DERIVED FROM SHELLFISH AND USE THEREOF |
Inventors:
Leverack Kennis Jay
Fluid Susan Jay A
Maramarro Jeffrey
Murrah Kyle Manman Bershire
Fluid Susan Jay A
Maramarro Jeffrey
Murrah Kyle Manman Bershire
Application Number:
JP51632196A
Publication Date:
August 13, 2008
Filing Date:
November 15, 1995
Export Citation:
Assignee:
Appraera Corporation
International Classes:
C12N15/09; C07H21/00; C12Q1/68; C12N15/00
Domestic Patent References:
| JP62157570A | ||||
| JP5199899A |
Foreign References:
| EP0601889A1 |
Other References:
Nucleic Acids Res.(1994)Vol.22,No.12、p.2424-2425
Nucleic Acids Res.(1993)Vol.21,No.16,p.3761-3766
Proc.Natl,Acad.Sci.USA(1991)Vol.88,No.16,p.7276-7280
Science(1993)Vol.260,p.778-783
Nucleic Acids Res.(1993)Vol.21,No.16,p.3761-3766
Proc.Natl,Acad.Sci.USA(1991)Vol.88,No.16,p.7276-7280
Science(1993)Vol.260,p.778-783
Attorney, Agent or Firm:
Minoru Nakamura
Fumiaki Otsuka
Sadao Kumakura
Shishido Kaichi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo
Takaki Nishijima
Atsushi Hakoda
Kenji Asai
Fumiaki Otsuka
Sadao Kumakura
Shishido Kaichi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo
Takaki Nishijima
Atsushi Hakoda
Kenji Asai