Title:
SEPARATING METHOD OF UROKINASE AND MEDICIN CONTAING UROKINASE AS USEFUL COMPONENT
Document Type and Number:
Japanese Patent JPS53130485
Kind Code:
A
Abstract:
Sepn. of a urokinase with a mol. wt. of ca. 54,000 (urokinase II) and a fraction contg. a urokinase with a mol. wt. of ca. 33,000 (urokinase I) from mixts. contg. them is effected by chromatographing a buffer soln. of the mixt. on an ion exchange resin, eluting with a concn. gradient of buffer salt. Also claimed is a pharmaceutical compsn. contg. urokinase II and 1 wt. % urokinase I. Since urokinase II has higher activity than I w.r.t. natural glutamyl-plasminogen and is equally active w.r.t. lysyl-plasminogen, it can be used as a thrombolytic agent at lower doses than conventional mixts. of I and II.
Inventors:
JIYAN KUROODO RORUMO
JIYAN SHIYOAI
SHIMON GURAI
JIYAN SHIYOAI
SHIMON GURAI
Application Number:
JP4258078A
Publication Date:
November 14, 1978
Filing Date:
April 11, 1978
Export Citation:
Assignee:
CHOAY SA
International Classes:
C12N9/72; A61K38/00; (IPC1-7): A61K37/48; C07G7/02; C07G7/026