PURPOSE: To obtain high-purity arginine in good yield by simple operation, by treating arginine containing impurities with a strong acidic cation exchange resin using ion exclusion chromatography.
CONSTITUTION: An aqueous solution containing impurities consisting essentially of at least one or two or more of lysine, ornithine, citrulline, histidine, sulfate radicals, chloride ions and coloring matter is adjusted to a pH of isoelectric point of the arginine or a pH close thereto. The resultant aqueous solution is then injected into a column filled with a strong acidic cation exchange resin, e.g. XFS-43279, and water is passed through the column to elute and give the aimed substance. The reaction is carried out by using the strong acidic cation resin in an amount of 4W5 times based on the amount of the aqueous solution when the arginine concentration of the aqueous solution is about 10% and impurity concentration thereof is about 1%.
NISHI AKIO
NARUSE MASAYOSHI
JPS58210027A | 1983-12-07 |