PURPOSE: To obtain the titled compound by a simple device by easy operations industrially advantageously, by bringing a solution comprising vitamin B12 containing admixtures into contact with a divinylbenzene/syrene copolymer resin, adsorbing vitamin B12 to the resin, washing it with pure water at a specific temperature, eluting it with an eluent.
CONSTITUTION: A product containing vitamin B12 obtained by cultivating a strain of Propionibacterium shermanii IFO12391 capable of producing vitamin B12 is centrifuged, and filtered to give a mold. Vitamin B12 together with admixtures is extracted from the mold with water at 80°C, to give an aqueous solution of crude vitamin B12. The solution comprising vitamin B12 containing admixtures is brought into contact with a divinylbenzene/styrene copolymer resin, vitamin B12 is adsorbed on the resin, the resin is washed with pure water or a solution with low concentration of an acid in pure water at 30W70°C, adsorbed vitamin B12 is eluted with an elutant, and an active eluate fraction is collected to give the desired high-purity compound.
KOJIMA ICHIROU
OOGUCHI YUTAKA
