To provide a sequentially fractional liquid chromatography apparatus which can shorten the period of a purification process cycle, minimize stationary phase carries, and simplify a pre-treatment of a mixture solution as a starting material.

A sequentially fractional liquid chromatography apparatus has a configuration such that a continuous stationary phase 6 comprising a band-like cyclic structure with dipping property goes through tanks having four functions with special structures, which are sequentially arranged in the order of an absorption tank 1, a washing tank 2, a dissociation tank 3, and a regenerating tank 5.