Title:
平面導波路を使用する1塩基多型の検出法
Document Type and Number:
Japanese Patent JP2005519593
Kind Code:
A
Abstract:
Methods and apparatus for detecting single nucleotide polymorphisms in genes of interest are disclosed. A plurality of probes is immobilized on a planar waveguide. The probes comprise sequences complementary to a wildtype sequence of the gene of interest and complementary to a sequence of a known SNP in the gene of interest. A fluorescently-labeled analyte is flowed over the planar waveguide. The binding between the labeled analyte and each of the probes causes a change in the fluorescence signal. The SNP is detected by comparing the hybridization kinetics of the analyte with each of the probes. A method of detecting single nucleotide polymorphisms in a gene of interest by sequencing by hybridization is also disclosed.
Inventors:
Heron, James N.
Tory, Samuel E.
One, Shu-Kun
Tory, Samuel E.
One, Shu-Kun
Application Number:
JP2003562606A
Publication Date:
July 07, 2005
Filing Date:
January 17, 2003
Export Citation:
Assignee:
UNIVERSITY OF UTHA RESEARCHFOUNDATION
International Classes:
C12N15/09; C12Q1/68; G01N33/50; (IPC1-7): C12Q1/68; C12N15/09; G01N33/50
Attorney, Agent or Firm:
Kazuo Shamoto
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Norihiro Fukasawa
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Norihiro Fukasawa