To provide a new apparatus for estimating solubility of protein developed by using a coli bacillus or wheat germ.
A solubility estimation device 1 is provided with: a statistical model storage part 18 that stores a statistical model generated by machine-learning the results of the development experiments of protein for determining whether protein is soluble or not, by using (1) the number of a predetermined amino acids included in the amino-acid sequence of protein, (2) the number of amino acids having predetermined physical features or chemical features, (3) the number of continuous predetermined amino acids, (4) the number of continuous amino acids having predetermined physical features or chemical features, (5) the number of film through-regions, (6) the number of predetermined amino acids in a surface residue, and (7) the ratio of a disorder region included in the protein, as parameters; a DNA sequence input part 10 for inputting a DNA sequence; a parameter value calculation part 12 for calculating the values of the parameters (1) to (7); and a solubility determination part 16 for determining the solubility of protein by applying the values of the parameters to a statistical model.
COPYRIGHT: (C)2011,JPO&INPIT
Yasushi Noguchi
Naoki Goshima
Yoshifumi Kawamura
Yu Kuroda
Bio industry informatization consortium
National University Corporation Tokyo University of Agriculture and Technology
| JP2005526518A | ||||
| JP2003238587A |
廣瀬 修一 外9名,大腸菌発現系におけるタンパク質発現・可溶化に関連している要因の解析,第9回 日本蛋白質科学会年会 プログラム・要旨集,日本,2009年 4月24日,p.83
Koji Morita
Reiko Tanaka
Mamoru Suzuki