Title:
空間分解リガンド−受容体結合アッセイ
Document Type and Number:
Japanese Patent JP6495382
Kind Code:
B2
Abstract:
A method for determining the concentration of an antigen in a sample comprising the steps of(a) combining in a reaction mixture (i) a sample suspected of containing an antigen, (ii) a capture antibody attached to a microparticle, which capture antibody specifically binds to the antigen, and (iii) a fluorescently labeled detection antibody which specifically binds to the antigen, and allowing formation of a complex comprising the microparticle attached to the capture antibody, the antigen, and the detection antibody;(b) acquiring a white light image of the reaction mixture in order to determine the location of the microparticle in the reaction of step (a) and a fluorescence image of the reaction mixture in order to determine the location of the fluorescently labeled detection antibody in the reaction of step (a);(c) selecting at least one region of interest from the images acquired in step (b), wherein the at least one region of interest is a region from which light signals emanate from the complex formed in step (a);(d) selecting pixels in the at least one region of interest for analysis;(e) calculating and recording the average and variance of the counts per pixel for the pixels selected in step (d), wherein the counts per pixel is the number of photons counted per pixel per unit of time;(f) omitting pixels that have counts greater or less than a specified variance;(g) calculating average counts per pixel of the remaining pixels; and(h) determining the concentration of the antigen from the data in step (g).
Inventors:
Rwan Chiao Chiao
Sylvia Sea Saldana
Joseph Joseph Skinner
Sergey Wai Techin
Sylvia Sea Saldana
Joseph Joseph Skinner
Sergey Wai Techin
Application Number:
JP2017130111A
Publication Date:
April 03, 2019
Filing Date:
July 03, 2017
Export Citation:
Assignee:
ABBOTT LABORATORIES
International Classes:
G01N33/543; G01N21/64; G01N21/78; G01N33/53
Domestic Patent References:
| JP2005508495A | ||||
| JP8334511A | ||||
| JP2011516863A |
Foreign References:
| US20060062440 |
Attorney, Agent or Firm:
Kawaguchi International Patent Office