Title:
分析エレメントによる核酸の種特異的検出
Document Type and Number:
Japanese Patent JP4219085
Kind Code:
B2
Abstract:
Detecting nucleic acid (I) on an analytical element (AE) that comprises a sample application zone (AZ) and a detection zone (DZ), and allows liquid transport between AZ and DZ, involves applying (I)-containing sample to AZ and detecting (I) in DZ by hybridization with a detectable probe (II). The new feature is that (I) is denatured on AE. Independent claims are also included for the following: (1) AE that is impregnated with a denaturing agent (III) for (I); and (2) a kit for detecting (I) comprising an AE and other reagents for detection.
Inventors:
Volker Keller
Andreas Low Cher
Joachim Steinbiss
Reiner Schrieffenbacher
Jürgen Klepp
Andreas Low Cher
Joachim Steinbiss
Reiner Schrieffenbacher
Jürgen Klepp
Application Number:
JP2000358273A
Publication Date:
February 04, 2009
Filing Date:
November 24, 2000
Export Citation:
Assignee:
Roche Diagnostics Gaem Behr
International Classes:
C12Q1/68; C12M1/00; G01N33/53; C12N15/09; C12Q1/6813; G01N33/543; G01N33/566
Domestic Patent References:
| JP6396559A | ||||
| JP1502317A | ||||
| JP61145459A | ||||
| JP347097A | ||||
| JP11509001A | ||||
| JP63245698A | ||||
| JP9504910A |
Other References:
Nucleic Acids Research,19(1991)p.5143-5151
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Ishii Teiji
Satoshi Fujita