核酸増幅の特異的抑制方法 - National University Corporation Osaka University

Title:

核酸増幅の特異的抑制方法

Document Type and Number:

Japanese Patent JP6964900

Kind Code:

B2

Abstract:

To provide a method for specifically inhibiting nucleic acid amplification which can be executed simply and at a low cost, and is capable of specifically inhibiting nucleic acid amplification in a target region without preventing nucleic acid amplification in a non-target region.SOLUTION: A method for specifically inhibiting nucleic acid amplification in a target region in template-dependent nucleic acid amplification reaction, where nucleic acid amplification in a plurality of regions progresses in the same reaction system, includes a process of adding single-stranded nucleic acid to be hybridized to the target region to a reaction system, in which the single-stranded nucleic acid is chimera between RNA or RNA and another nucleic acid, and consists of 10 to 200 bases.SELECTED DRAWING: None

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Inventors:

Fujii Hodaka
Toshiji Fujita
Naoki Tanikawa

Application Number:

JP2020077741A

Publication Date:

November 10, 2021

Filing Date:

April 24, 2020

Export Citation:

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Assignee:

National University Corporation Osaka University

International Classes:

C12N15/10

Other References:

Nucleic Acid Research,2001, Vol.29, No.6,e31

Attorney, Agent or Firm:

Ryu Iwatani

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