Title:
STABILIZATION OF PROTEIN
Document Type and Number:
Japanese Patent JP3892954
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for easily imparting organic solvent resistance to a protein unstable to organic solvents.
SOLUTION: This method for stabilizing a protein comprises adding a protein folding factor to a solution containing an organic solvent (e.g. ethanol) and containing a protein (e.g. β-galactosidase, peroxidase) to effect inhibition of the protein in the solution from being deactivated due to the organic solvent; wherein the protein folding factor is a protein having molecular chaperone activity (e.g. GroE) or peptidyl prolyl-cis, trans-isomerase which is expressed from a DNA derived from Methanococcus thermolithotrophicus.
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Inventors:
Akira Ideno
Atsushi Doi
Atsushi Doi
Application Number:
JP25093097A
Publication Date:
March 14, 2007
Filing Date:
September 16, 1997
Export Citation:
Assignee:
Sekisui Chemical Co.,Ltd.
International Classes:
C07K1/113; C12N15/09; C07K14/195; C12N9/48; C12N9/90; C12N15/00; C12R1/01; (IPC1-7): C07K1/113; C12N9/48; C12N9/90; C12N15/09; //C07K14/195; (C12N9/48; C12R1:01); (C12N9/90; C12R1:01); (C12N15/09; C12R1:01)
Domestic Patent References:
| JP7067641A | ||||
| JP7048398A |
Other References:
The Journal of Biological Chemistry, 1992, vol.267, p.17773-17779
The EMBO Journal, 1995, vol.14, p.4939-4948
J. Mol. Biol., 1997 Sep. 5, vol.271, p.827-837
日本Archaebacteria研究会講演会要旨集, 1995, vol.8, p.31-32
The EMBO Journal, 1995, vol.14, p.4939-4948
J. Mol. Biol., 1997 Sep. 5, vol.271, p.827-837
日本Archaebacteria研究会講演会要旨集, 1995, vol.8, p.31-32