Title:
STABLE AND SOLUBLE ANTIBODIES THAT INHIBIT VEGF
Document Type and Number:
Japanese Patent JP2019162127
Kind Code:
A
Abstract:
To provide stable and soluble antibodies that inhibit VEGF.SOLUTION: The invention relates to a soluble and stable anti-VEGF immunobinder comprising a CDR from a rabbit monoclonal antibody. The antibody is designed for diagnosis and/or treatment of VEGF-mediated disorders. A hybridoma, nucleic acid vector and host cell for expressing the recombinant antibody of the invention, a method for isolating them and the use of the antibody in medicine are also disclosed. In one embodiment, the invention provides an immunobinder that binds to VEGF so that suitable for blocking the function of VEGF in vivo. The CDR of these immunobinders is derived from a rabbit anti-VEGF monoclonal antibody obtained from a rabbit immunized with human VEGF and/or a fragment having a specific sequence thereof.SELECTED DRAWING: None
Inventors:
LEONARDO BORRAS
DAVID URECH
TEA GUNDE
DAVID URECH
TEA GUNDE
Application Number:
JP2019090735A
Publication Date:
September 26, 2019
Filing Date:
May 13, 2019
Export Citation:
Assignee:
ESBATECH A NOVARTIS CO LLC
International Classes:
C12N15/13; A61K39/395; A61P7/10; A61P9/10; A61P17/06; A61P19/02; A61P27/02; A61P27/06; A61P29/00; A61P35/00; A61P43/00; C07K16/22; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/06; C12N15/63; C12P21/08
Domestic Patent References:
| JP2007520991A | 2007-08-02 | |||
| JP2003527334A | 2003-09-16 | |||
| JP2008518619A | 2008-06-05 |
Other References:
J. VET. DIAGN. INVEST., vol. 17, JPN6014000189, 2005, pages 346 - 350, ISSN: 0004631015
J. BIOL. CHEM., 2006, 281(10), PP.6625-6631, JPN6014000193, ISSN: 0004631016
J. MOL. BIOL., vol. 1992, 224, JPN6013015126, pages 487 - 499, ISSN: 0004631017
J. BIOL. CHEM., 2000, 275(18), PP.13668-13676, JPN6014000187, ISSN: 0004631018
J. MOL. BIOL., 2001, 309(3), PP.657-670, JPN6014000196, ISSN: 0004631009
J. CANCER RES. CLIN. ONCOL., 1998, 124(11), PP.615-620, JPN6014000178, ISSN: 0004631010
BIOCHEM. BIOPHYS. RES. COMMUN., 2001, 281(2), PP.562-568, JPN6014000179, ISSN: 0004631011
ANGLE ORTHOD., 2005, 75(5), PP.797-804, JPN6014000182, ISSN: 0004631012
J. BIOL. CHEM., 2000, 275(46), PP.36073-36078, JPN6014000185, ISSN: 0004631013
AM. J. CLIN. PATHOL., vol. 124, JPN6014000188, 2005, pages 295 - 302, ISSN: 0004631014
J. BIOL. CHEM., 2006, 281(10), PP.6625-6631, JPN6014000193, ISSN: 0004631016
J. MOL. BIOL., vol. 1992, 224, JPN6013015126, pages 487 - 499, ISSN: 0004631017
J. BIOL. CHEM., 2000, 275(18), PP.13668-13676, JPN6014000187, ISSN: 0004631018
J. MOL. BIOL., 2001, 309(3), PP.657-670, JPN6014000196, ISSN: 0004631009
J. CANCER RES. CLIN. ONCOL., 1998, 124(11), PP.615-620, JPN6014000178, ISSN: 0004631010
BIOCHEM. BIOPHYS. RES. COMMUN., 2001, 281(2), PP.562-568, JPN6014000179, ISSN: 0004631011
ANGLE ORTHOD., 2005, 75(5), PP.797-804, JPN6014000182, ISSN: 0004631012
J. BIOL. CHEM., 2000, 275(46), PP.36073-36078, JPN6014000185, ISSN: 0004631013
AM. J. CLIN. PATHOL., vol. 124, JPN6014000188, 2005, pages 295 - 302, ISSN: 0004631014
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Natsuki Morishita