PURPOSE: Immobilize lactase is sterilized and washed by use of an aqueous solution containing antimicrobials, an acidic aqueous solution and an almost neutral aqueous solution to achieve the purpose without the deactivation of the lactase.
CONSTITUTION: A lactase originating from Aspergillus oryzae is immobilized on a support or inclusively coagulating substance through covalent bonds or adsorbed on an ion exchange resin with micropores to prepare immobilized lactase. The resultant immobilized lactase is used to decompose lactose in a solution containing the same, then sterilized and washed. The sterilization and washing are effected by an appropriate combination selected from sterilization with a solution of pH 2W8.5 containing antimicrobials, sterilization with antimicrocidal solution of 2W4pH, sterilization with an aqueous solution of 6W8.5 pH containing both a detergent and anti- microbials are washing with water.
YAMAMOTO HIDEFUMI
KOUNO EMIKO
HATSUTORI MAMORU
YAMAGUCHI HISAO
SHIN NIHON KAGAKU KOGYO KK
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