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Title:
SUBSTANCE CONTAINING LACTOSUCROSE IN HIGH CONTENT AND ITS PRODUCTION AND USE
Document Type and Number:
Japanese Patent JPH1057092
Kind Code:
A
Abstract:

To enable easy and mass production of the subject substance- containing solution by allowing a glycosyltransferase to act on an aqueous solution containing sucrose and lactose and removing contaminant saccharides from the resultant lactosucrose containing aqueous solution.

A glycosyltansferase (for example, fructose glycosy-transferase) is allowed to act on an aqueous solution containing sucrose and fructose. Then, the contaminants are removed from the resultant lactosucrose-containing saccharide solution, preferably by alkali or alkaline earth metal type storing acid cationic ion-exchange resin column chromatography using only water to give a solution containing more than 45w/w% of lactosucrose and the solution is collected. usually, the glysyltransferase is allowed to act on an aqueous solution containing sucrose and lactose at a ratio of 1/10-10/1 in the total saccharide concentration of 10-60wt.% at a pH of 3-9 and a temperature of 5-70°C.


Inventors:
HARA KOZO
FUJITA TAKATERU
YAMASHITA MASAYUKI
TSUNETOMI YASUHIKO
SAKAI SHUZO
MIYAKE TOSHIO
Application Number:
JP10543797A
Publication Date:
March 03, 1998
Filing Date:
April 09, 1997
Export Citation:
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Assignee:
HAYASHIBARA BIOCHEM LAB
BIOFERMIN PHARMA
ENSUIKO SUGAR REFINING
International Classes:
A23L2/52; A23L2/56; A23L2/58; A23L2/60; A23L2/66; A23L2/78; A23L2/84; A23L27/30; A61K31/70; A61K31/7016; A61K33/06; A61K33/42; A61K47/26; A61P1/00; A61P1/12; A61P3/00; A61P3/02; A61P3/08; A61P7/00; A61P9/12; A61P15/00; A61P31/00; A61P35/00; A61P43/00; C07H3/04; C12P19/18; C12S3/02; C99Z99/00; C12R1/865; (IPC1-7): C12P19/18; A23L1/236; A23L1/30; A23L2/52; A23L2/60; A61K31/70; A61K33/06; A61K33/42; A61K47/26; C12S3/02
Attorney, Agent or Firm:
Mitsuo Suma