Title:
SUGAR CHAIN ANALYSIS METHOD, SUGAR CHAIN ANALYSIS SYSTEM, SUGAR CHAIN ANALYSIS PROGRAM, AND SUGAR CHAIN ANALYSIS KIT
Document Type and Number:
Japanese Patent JP2019148564
Kind Code:
A
Abstract:
To identify a variety of sugar chains with improving the throughput of measurement.SOLUTION: After fluorescently labeling a sample of a sugar chain mixture (S2), fluorescence detection by separating it by a microchip electrophoresis method under a plurality of types of buffers with the addition of buffer and mutually different lectins without addition of lectins respectively (S3). A high concentration of gel with which a molecular sieve effect is obtained is used as the buffer. A plurality of electroferrograms are created based on the detection results (S4). The peak corresponding to the sugar chain is substantially lost since the movement of the sugar chain to which lectin is specifically bound is delayed. Therefore, the sugar chain is identified by estimating the structure of the sugar chain in the sample (S5) based on the presence or absence of peaks on the type of the lectin and a plurality of electroferrograms. The types of the sugar chains that can be identified also increases since the number of the types of available lectins greatly increases by using a high concentration of appropriate types of gel in the buffer. In addition, it is possible to improve the throughput because the measurement time is short compared to the capillary electrophoresis.SELECTED DRAWING: Figure 1
More Like This:
Inventors:
KINOSHITA MITSUHIRO
Application Number:
JP2018035083A
Publication Date:
September 05, 2019
Filing Date:
February 28, 2018
Export Citation:
Assignee:
UNIV KINKI
SHIMADZU CORP
SHIMADZU CORP
International Classes:
G01N27/447
Domestic Patent References:
| JP2009074811A | 2009-04-09 | |||
| JP2002243701A | 2002-08-28 | |||
| JP2009508475A | 2009-03-05 |
Foreign References:
| WO2004036216A1 | 2004-04-29 | |||
| WO2002044708A1 | 2002-06-06 | |||
| US5543054A | 1996-08-06 |
Other References:
掛樋一晃、松野裕樹、木下充弘: "キャピラリー電気泳動による蛍光標識糖鎖の超高感度分析", 臨床化学, vol. 34, no. 4, JPN7021002199, 30 November 2005 (2005-11-30), pages 326 - 335, ISSN: 0004683939
木下充弘、掛樋一晃: "糖鎖解析におけるキャピラリー電気泳動が果たす役割", 生物物理化学, vol. 52, no. 3, JPN7021002200, 15 September 2008 (2008-09-15), pages 111 - 116, ISSN: 0004683940
木下充弘、掛樋一晃: "糖鎖解析におけるキャピラリー電気泳動が果たす役割", 生物物理化学, vol. 52, no. 3, JPN7021002200, 15 September 2008 (2008-09-15), pages 111 - 116, ISSN: 0004683940
Attorney, Agent or Firm:
Kyoto International Patent Office