To obtain a surfactant or cleansing agent having a strong cleansing power based on microorganism and enzyme action without using chemicals, and a wide range in treated products to be cleansed and easy in handling.

This microorganism culture solution-containing cleansing agent in which (a) an aerobic microorganism group, (b) an anaerobic microorganism group, (c) at least one kind of Basidiomycetes belonging to Pleurotus osreatus family and (d) photosynthetic bacteria live together contains the enzyme derived from metabolites of the microorganisms thereof and carbon splitting enzyme. The carrier containing an active ingredient of the culture solution and based on micronized carbonaceous matter is used as the cleansing agent.