Title:
SYNTHETIC CHLOROPLAST TRANSIT PEPTIDES
Document Type and Number:
Japanese Patent JP2019047803
Kind Code:
A
Abstract:
To provide a synthetic chloroplast transit peptide that is useful in the heterologous expression of protein targeted by the chloroplast in a higher plant.SOLUTION: Provided is a nucleic acid molecule comprising a polynucleotide encoding a chimeric polypeptide comprising a synthetic chloroplast transit peptide and a polypeptide of interest, when the polynucleotide is expressed in plant cells, the polypeptide of interest being targeted by a chloroplast in the plant cells, the polynucleotide being a first nucleotide sequence encoding the synthetic CPT, each amino acid residue of the synthetic CPT being identical to the amino acid residue at the same position of 3-enolpyruvylshikimate-5-phosphoric acid synthetase (EPSPS), and the nucleic acid molecule comprising a second nucleotide sequence encoding the polypeptide of interest that is operably linked to the first nucleotide sequence in frame.SELECTED DRAWING: None
Inventors:
JUSTIN M LIRA
ROBERT M CICCHILLO
CARLA YERKES
ANDREW E ROBINSON
ROBERT M CICCHILLO
CARLA YERKES
ANDREW E ROBINSON
Application Number:
JP2018207345A
Publication Date:
March 28, 2019
Filing Date:
November 02, 2018
Export Citation:
Assignee:
DOW AGROSCIENCES LLC
International Classes:
C12N15/62; A01H1/00; A01H5/00; C07K19/00; C12N5/10
Domestic Patent References:
| JP2001197841A | 2001-07-24 | |||
| JPH06500472A | 1994-01-20 |
Foreign References:
| US20070295251A1 | 2007-12-27 | |||
| US20100071090A1 | 2010-03-18 | |||
| US20110107446A1 | 2011-05-05 | |||
| US20030188336A1 | 2003-10-02 | |||
| WO2008105890A2 | 2008-09-04 |
Attorney, Agent or Firm:
Hiroshi Kobayashi
Eiji Katayama
Norio Omori
Hakoda Mitsuru
Yasuhito Suzuki
Eiji Katayama
Norio Omori
Hakoda Mitsuru
Yasuhito Suzuki