NEW MATERIAL: A synthetic peptide having an amino acid sequence of the region 1-42, 181-211 or 212-235 of the S1 subunit of pertussis toxin from Bordetella pertussis, the peptide being capable of stimulating T-cellular immunity to pertussis toxin.
USE: Useful as an immunogen-stimulating agent for anti-pertussis, and as acellular anti-pertussis vaccines.
PROCESS: For example, T-cells are cultivated in a medium supplemented with S1 subunit of pertussis toxin. A clone producing an anti-S1 antibody is selected and cultivated to separate an anti-S1 antibody. The reactive portion of the antibody is identified, and a peptide having an amino acid sequence corresponding to the S1 fragment is synthesized by Merriefield solid phase synthesis. Thus, a synthetic peptide containing an amino acid sequence of the amino acid residues of the partial region of the S1 subunit of pertussis toxin can be obtained.
ANTONETSURA BARUTOROONI
RINO RATSUPUOORI
ARUDO TARIABUE
