Title:
選択マーカー遺伝子のない ＤＮＡベクターを用いる遺伝子治療方法
Document Type and Number:
Japanese Patent JPH09512030
Kind Code:
A
Abstract:
The use of a circular vector DNA to produce a pharmaceutical agent for the treatment of mammals or humans by gene therapy wherein the vector DNA contains a selection marker gene and a DNA sequence that is heterologous for the vector which causes a modulation, correction or activation of the expression of an endogenous gene or the expression of a gene introduced into the cells of the mammal or the human by the vector DNA which is characterized in that the vector nucleic acida) is amplified under selection pressure and cleaved in such a way that the selection marker gene and the heterologous DNA are present on separate DNA fragments,b) the DNA fragment which contains the heterologous DNA or both fragments are recircularized to form vectors,c) the DNA fragments are separated before or after the recircularizationd) the recircularized DNA fragment which contains the heterologous DNA is isolated ande) the recircularized DNA fragment obtained in this manner is used to produce the pharmaceutical agent.
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Inventors:
ゼーバー，シュテーファン
リュガー，リュディガー
リュガー，リュディガー
Application Number:
JP50696696A
Publication Date:
December 02, 1997
Filing Date:
July 31, 1995
Export Citation:
Assignee:
ベーリンガー マンハイム ゲゼルシャフト ミット ベシュレンクテル ハフツング
International Classes:
C12N15/09; A61K9/12; A61K35/76; A61K38/00; A61K48/00; A61P1/00; A61P11/00; A61P17/00; C07H21/04; C07K14/47; C12N15/10; C12N15/63; C12N15/65; C12N15/85; (IPC1-7): A61K48/00; A61K9/12; A61K35/76; A61K38/00; A61K48/00; C07H21/04; C12N15/09
Attorney, Agent or Firm:
石田 敬 （外３名）