Title:
熱安定性ＣＡＳ９ヌクレアーゼ
Document Type and Number:
Japanese Patent JP7088528
Kind Code:
B2
Abstract:
Thermostable Cas9 nucleases. The present invention relates to the field of genetic engineering and more particularly to nucleic acid editing and genome modification. The present invention provides in isolated Cas protein or polypeptide fragment thereof having an amino acid sequence of SEQ ID NO: 1 or a sequence of at least 77% identity therewith, wherein the Cas protein or polypeptide is capable of DNA cleavage at a temperature in the range 50° C. and 100° C. inclusive. The invention further provides isolated nucleic acid molecules encoding said Cas9 nucleases, expression vectors and host cells. The Cas9 nucleases disclosed herein provide novel tools for genetic engineering at elevated temperatures and are of particular value in the genetic manipulation of thermophilic organisms; particularly microorganisms.
Inventors:
Worst, van der, john
Darth, Martinas Johannes Arnoldas
Köngen, Selvatius Wilhelms Maria
Voss, de, Willem Meindert
Darth, Martinas Johannes Arnoldas
Köngen, Selvatius Wilhelms Maria
Voss, de, Willem Meindert
Application Number:
JP2017564480A
Publication Date:
June 21, 2022
Filing Date:
June 06, 2016
Export Citation:
Assignee:
Wageningen Universitet
International Classes:
C12N15/55; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/16; C12N15/09; C12N15/62; C12N15/63; C12N15/65
Foreign References:
| US20140068797 |
Other References:
Database DDBJ/EMBL/GenBank [online], Accession No. ESU73649.1,
Cell (2013) Vol.154, pp.442-451
Genome Announcements (2014) Vol.2, No.3, e00559-14, pp.1-2
Database DDBJ/EMBL/GenBank [online], Accession No. ESU73649.1,
Cell (2013) Vol.154, pp.442-451
Cell (2013) Vol.154, pp.442-451
Genome Announcements (2014) Vol.2, No.3, e00559-14, pp.1-2
Database DDBJ/EMBL/GenBank [online], Accession No. ESU73649.1,
Cell (2013) Vol.154, pp.442-451
Attorney, Agent or Firm:
Hiroshi Kobayashi
Norio Omori
Fujita Nao
Yasuhito Suzuki
Norio Omori
Fujita Nao
Yasuhito Suzuki