Title:
THERMOSENSITIVE PLASMID
Document Type and Number:
Japanese Patent JP2004166712
Kind Code:
A
Abstract:
To provide a plasmid usable for genetically modifying bacteria having Gram-positive dye-affinity, especially, industrially and medically important lactobacillus.
The bacterial vector plasmid has an efficient replication origin in Gram-positive bacteria. The plasmid has at least one marker gene which expresses itself in a bacterial host strain, an efficient replication system which is thermosensitive based on a temperature compatible with the viability of the host strain, and the temperature of replication inhibition below or equal to approximately 37°C.
Inventors:
ALEXANDRA GRUSS
EMMANUELLE MAGUIN
EMMANUELLE MAGUIN
Application Number:
JP2004038967A
Publication Date:
June 17, 2004
Filing Date:
February 16, 2004
Export Citation:
Assignee:
AGRONOMIQUE INST NAT RECH
International Classes:
C12N1/21; C12N15/74; C12N15/09; C12N15/90; (IPC1-7): C12N15/09; C12N1/21
Attorney, Agent or Firm:
Kenji Yoshitake
Yukitaka Nakamura
Konno Akio
Noritaka Yokota
Yukitaka Nakamura
Konno Akio
Noritaka Yokota