Title:
THERMOSTABLE MALTOSE PHOSPHORYLASE, ITS PRODUCTION, BACTERIUM USED THEREFOR AND USE OF THE SAME ENZYME
Document Type and Number:
Japanese Patent JP3691875
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain the subject new enzyme by culturing a bacterium belonging to the genus Bacillus, capable of producing maltose phosphorylase, having thermostability, carrying out enzyme reaction at a high reaction temperature, reducing contamination with various germs, providing trehalose in a short time.
SOLUTION: This new enzyme is obtained by culturing a bacterium belonging to the genus Bacillus [e.g. Bacillus sp.MK-1 (FERM P-15,045) etc.], capable of producing maltose phosphorylase, in a nutrient medium and collecting the product from the culture product, provides equimolar amounts of glucose and β-glucose-1-phosphate when treated with maltase in the presence of phosphoric acid, forms equimolar amounts of maltose and phosphoric acid when treated with glucose and β-glucose-1-phosphate, has activity by treatment in 10mM acetic acid buffer solution (pH6.0) at 60°C for 15 minutes, equal to or higher than 80% activity of nontreatment, 55-70°C optimum temperature, optimum pH6.0 to pH7.0, 150,000-190,000 molecular weight measured by gel filtration and industrially and advantageously provides β-glucose-1-phosphate, trehalose, etc.
Inventors:
Keiko Ishii
Inoue Yasushi
Tetsuji Tomita
Inoue Yasushi
Tetsuji Tomita
Application Number:
JP21300595A
Publication Date:
September 07, 2005
Filing Date:
July 31, 1995
Export Citation:
Assignee:
Showa Sangyo Co., Ltd.
International Classes:
C12N9/12; C12N1/20; C12N9/10; C12P19/12; C12P19/18; C12R1/07; (IPC1-7): C12N9/12; C12N1/20; C12P19/12
Domestic Patent References:
| JP8131157A | ||||
| JP8131182A | ||||
| JP8280382A | ||||
| JP191778A | ||||
| JP5480488A |
Attorney, Agent or Firm:
Noboru Sakaguchi
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