Title:
【発明の名称】遺伝子導入植における組換メッセンジャーRNAのウィルス増幅関連出願のクロスリファレンス
Document Type and Number:
Japanese Patent JPH08505289
Kind Code:
A
Abstract:
A novel method of over expressing genes in plants is provided. This method is based on the RNA amplification properties of plus strand RNA viruses of plants. A chimeric multicistronic gene is constructed containing a plant promoter, viral replication origins, a viral movement protein gene, and one or more foreign genes under control of viral subgenomic promoters. Plants containing one or more of these recombinant RNA transcripts are inoculated with helper virus. In the presence of helper virus recombinant transcripts are replicated producing high levels of foreign gene RNA. Sequences are provided for the high level expression of the enzyme chloramphenicol acetyltransferase in tobacco plants by replicon RNA amplification with helper viruses and movement protein genes derived from the tobamovirus group.
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Inventors:
Turpen, Thomas H.
Application Number:
JP51608094A
Publication Date:
June 11, 1996
Filing Date:
December 29, 1993
Export Citation:
Assignee:
Biosource Genetics Corporation
International Classes:
C07K14/08; C12N5/10; C12N15/09; C12N15/12; C12N15/40; C12N15/82; A01H5/00; C12N15/83; C12P21/02; C12R1/92; (IPC1-7): C12N15/09; A01H5/00; C12P21/02
Attorney, Agent or Firm:
Takashi Ishida (3 others)