Title:
【発明の名称】シクロヘキセン核酸
Document Type and Number:
Japanese Patent JP2003519231
Kind Code:
A
Abstract:
Replacement of the furanose moiety of, for example, DNA by a cyclohexene ring gives a new nucleic acid structure: cyclohexene nucleic acids or CeNA. Cyclohexene nucleic acids can be obtained by the classical phosphoramidite chemistry starting from protected cyclohexenyl nucleoside building blocks. Incorporation of cyclohexenyl nucleosides in a DNA chain increases the stability of a DNA/RNA hybrid, the complex formed between cyclohexenyl oligoadenylate and its DNA or RNA complement is of similar stability. CD and NMR studies indicate easy conformational adaptation of a cyclohexenyl nucleoside when incorporated in a natural nucleic acid structure. CeNA is stable against degradation in serum and a CeNA/RNA hybrid is able to activate E. Coli Rnase H, resulting in cleavage of the RNA strand.
Inventors:
One, gin
Heldewein, pete
Heldewein, pete
Application Number:
JP2001550227A
Publication Date:
June 17, 2003
Filing Date:
December 27, 2000
Export Citation:
Assignee:
Car Yu Ruben Research and Development
International Classes:
G01N33/68; A61K9/08; A61K9/14; A61K31/712; A61P25/00; A61P43/00; C07D473/34; C07H19/16; C07H19/20; C07H21/00; C07H21/02; C07H21/04; C12N15/09; (IPC1-7): C07H19/16; A61K9/08; A61K9/14; A61K31/712; A61P25/00; A61P43/00; C07H19/20; C07H21/02; C07H21/04; C12N15/09; G01N33/68
Attorney, Agent or Firm:
Kazuo Shamoto (5 outside)