Title:
【発明の名称】T.バリアビリス由来のD-アミノ酸オキシダーゼプロモーター
Document Type and Number:
Japanese Patent JP2002505587
Kind Code:
A
Abstract:
A D-amino acid oxidase promoter isolated from T. variabilis has been incorporated into S. cerevisiae expression vectors to increase the expression of heterologous genes. The heterologous promoter has been placed upstream of selective markers, auxotrophic nutritional and/or dominant selectable markers. Because the promoter is from another yeast specie, it is weakly recognized by and S. cerevisiae. To compensate for the weak transcripts of selectable marker genes transcribed from the weak heterologous promoter, the plasmid containing the gene of the interest is amplified leading to a higher expression of protein of interest.
Inventors:
Liu, Suo W
Franceschini, Thomas
Franceschini, Thomas
Application Number:
JP50453199A
Publication Date:
February 19, 2002
Filing Date:
June 08, 1998
Export Citation:
Assignee:
Bristol-Myers Squibb Company
International Classes:
C07K14/495; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12N9/06; C12N9/16; C12N9/18; C12N9/86; C12N15/81; (IPC1-7): C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)