Title:
【発明の名称】DNA断片
Document Type and Number:
Japanese Patent JP2590885
Kind Code:
B2
Abstract:
PURPOSE:To provide a DNA fragment containing a base sequence coding a non-A, non-B hepatitis specific antigen protein and useful for the mass- production of a non-A, non-B hepatitis specific antigen protein by a recombinant DNA technique. CONSTITUTION:A liver tissue of a human or chimpanzee affected with non-A, non-B hepatitis is homogenized in an aqueous solution of guanidium thiocyanate and whole RNA is separated as a precipitate by an equilibrium density gradient ultracentrifugation using cesium chloride. The separated whole RNA is purified by the extraction with phenol and the precipitation with ethanol. The RNA is further purified by oligo(dT)-cellulose column chromatography to separate a poly(A)-containing RNA, which is used as a raw material for mRNA. The objective DNA is determined from the mRNA through a cDNA library. The DNA is composed of a sequence of 1,333 bases.
Inventors:
Kazu Takahashi Exhibition
Tatsuro Shibui
Michiru Uchida
Koubayashi Rie
Yutaka Teranishi
Nakanishi Shigetada
Naomi Kitamura
Tatsuro Shibui
Michiru Uchida
Koubayashi Rie
Yutaka Teranishi
Nakanishi Shigetada
Naomi Kitamura
Application Number:
JP14058687A
Publication Date:
March 12, 1997
Filing Date:
June 04, 1987
Export Citation:
Assignee:
Mitsubishi Chemical Corporation
International Classes:
C12N15/00; C07K14/18; C12N15/09; C12P21/02; C12R1/91; (IPC1-7): C12N15/09; C12P21/02
Attorney, Agent or Firm:
Hasegawa Moji