Title:
【発明の名称】イムノアッセイ法及びキット
Document Type and Number:
Japanese Patent JP2999747
Kind Code:
B2
Abstract:
An immunoassay procedure for detection of analytes in urine wherein an immunological reaction is conducted in an aqueous phase containing the urine and a filterable immunocomposite containing an inherently colored gold sol particle is formed if the assay is positive. The colored, gold sol particle containing immunocomposite is collected for direct visual observation on a filter element. False positive readings are minimized by (1) removing contaminants from the urine to prevent clogging of the filter element and non-specific binding of unreacted reactants thereto by contacting the urine with a porous contact element having a detergent material dispersed on its external and interstitial surfaces; (2) preventing non-specific binding of unreacted immunoreactive substances to the filter element by blocking the latter with polyvinyl pyrrolidone; and/or (3) including a green dye material in the aqueous reaction phase to improve the optical distinction after washing the collection filter between negative and positive results on the filter.
Inventors:
Charles C. Ditrow
Elle Edward Cannon
Francis X. Cole
Gene A. Davis
Eric C. Sigillo
Alicia G. Danti
Elle Edward Cannon
Francis X. Cole
Gene A. Davis
Eric C. Sigillo
Alicia G. Danti
Application Number:
JP9771298A
Publication Date:
January 17, 2000
Filing Date:
April 09, 1998
Export Citation:
Assignee:
Heijia Sciences, Incorporated
International Classes:
G01N33/543; G01N33/58; G01N33/53; (IPC1-7): G01N33/543; G01N33/543
Domestic Patent References:
JP60196653A | ||||
JP6298257A | ||||
JP61502214A |
Attorney, Agent or Firm:
Takashi Ishida (2 outside)