【発明の名称】リパーゼ変異体

Title:

【発明の名称】リパーゼ変異体

Document Type and Number:

Japanese Patent JP3469234

Kind Code:

B2

Abstract:

Lipases comprising a trypsin-like catalytic triad including an active serine located in a predominantly hydrophobic, elongated binding pocket of the lipase molecule, which pocket forms part of and is surrounded by a lipid contact zone, are mutated by deletion or substitution of one or more amino acid residues in the lipid contact zone so as to change the electrostatic charge and/or hydrophobicity of the lipid contact zone or so as to change the surface conformation of the lipid contact zone of the lipases.

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Inventors:

Alain Svensen
Clausen, Eve Grot
Patkal, Shamkant Anant
Erik, Golmsen

Application Number:

JP51573291A

Publication Date:

November 25, 2003

Filing Date:

September 13, 1991

Export Citation:

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Assignee:

Novozymes actiesel scab

International Classes:

C11D3/386; C11D7/42; C12N1/15; C12N1/21; C12N5/10; A01H5/00; C12N9/20; C12N15/09; C12N15/55; C12N15/62; C12N15/80; C12R1/125; C12R1/69; C12R1/865; (IPC1-7): C12N15/09; C11D3/386; C11D7/42; C12N1/15; C12N9/20

Domestic Patent References:

JP2225599A

Other References:

【文献】Davis RC et al.,Hepatic lipase:site-directed mutagenesis of a serine residue important for catalytic activity,J.Biol.Chem.,米国,1990年 4月15日,265(11)

Attorney, Agent or Firm:

Takashi Ishida (4 others)

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