Title:
【発明の名称】オキサロバクターを検出する材料および方法
Document Type and Number:
Japanese Patent JP2002508653
Kind Code:
A
Abstract:
The subject invention concerns the novel use of formyl-CoA transferase enzyme together with oxalyl-CoA decarboxylase enzyme for the detection and measurement of oxalate in biological samples. The use of the enzyme system according to the subject invention results in the conversion of oxalate into carbon dioxide and formate. Because the production of formate is directly correlated to the concentration of oxalate present in a sample, the determination of the resulting formate concentration provides an accurate, sensitive and rapid means for detecting even low levels of oxalate. The subject invention further concerns the cloning, sequencing and expression of the genes that encode the formyl-CoA transferase enzyme and the oxalyl-CoA decarboxylase enzyme of Oxalobacter formigenes. The subject invention also concerns methods for detecting the presence of Oxalobacter formigenes organisms in a sample, and the polynucleotide probes and primers used in the detection method.
Inventors:
Peck Amon Bee.
Sidu Hermit
Sidu Hermit
Application Number:
JP51835798A
Publication Date:
March 19, 2002
Filing Date:
September 23, 1997
Export Citation:
Assignee:
University of Florida
International Classes:
C12N15/09; C12N9/10; C12N9/88; C12Q1/04; C12Q1/48; C12Q1/527; C12Q1/68; C12R1/01; (IPC1-7): C12N15/09; C12Q1/68
Attorney, Agent or Firm:
Shimizu Hatsushi (1 person outside)