Title:
【発明の名称】血液成分の精製方法
Document Type and Number:
Japanese Patent JP2002528200
Kind Code:
A
Abstract:
A method for rapid purification of a blood component from blood is described in which the blood plasma is first separated from the cellular blood elements by any conventional means, such as centrifugation. An affinity cartridge is then activated with a molecule, such as an amino acid, which binds with a blood component such as plasminogen. The separated blood plasma is then passed through the affinity cartridge such that the blood component is retained by the affinity cartridge. Thereafter, the blood component is eluted from the affinity cartridge by passing a buffer solution containing a releasing agent through the affinity cartridge. This releasing agent disengages the blood component from the affinity cartridge. The releasing agent is then separated from the eluted solution by passing the eluted solution through a device, such as an ion exchange or size exclusion device. The separated blood component, e.g. plasminogen, is then converted to plasmin by adding a known amount of an enzyme to the solution from which the releasing agent has been removed.
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Inventors:
Trees Michael Tee
William George A
Harzer michael
Daily Wendlin A
William George A
Harzer michael
Daily Wendlin A
Application Number:
JP2000578091A
Publication Date:
September 03, 2002
Filing Date:
October 19, 1999
Export Citation:
Assignee:
Neuve Technologies, Inc.
International Classes:
A61M1/02; B01D15/04; B01D15/08; B01J20/32; C12N9/68; G01N30/00; G01N30/02; G01N30/04; A61K38/00; (IPC1-7): A61M1/02; B01D15/04; B01D15/08
Attorney, Agent or Firm:
Minoru Nakamura (9 outside)