【発明の名称】組換え宿主細胞を用いたタンパク質の調製法 - Merck Patent Gesellschaft Mitt Beschlenktel Haftung

Title:

【発明の名称】組換え宿主細胞を用いたタンパク質の調製法

Document Type and Number:

Japanese Patent JP3085700

Kind Code:

B2

Abstract:

The invention relates to a process for achieving high, thermally-induced expression rates, which last for a relatively long period of time, of proteins in recombinant cells, preferably in E. coli cells, by optimizing cell density, induction temperature and supplemented amino-acid-containing substrates.

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Inventors:

Martin Heinrich
Wolfgang Brumer
Kurt Brov

Application Number:

JP29556090A

Publication Date:

September 11, 2000

Filing Date:

November 02, 1990

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Assignee:

Merck Patent Gesellschaft Mitt Beschlenktel Haftung

International Classes:

C12N9/00; C12N1/00; C12N1/21; C12N9/04; C12N15/09; C12N15/53; C12N15/67; C12N15/73; C12P1/00; C12P21/00; C12P21/02; C12R1/19; (IPC1-7): C12N15/09; C12N9/04; C12P21/00; C12P21/02

Domestic Patent References:

JP6312283A
JP6420098A

Other References:

Ｊ．Ｂｉｏｔｅｃｈ．，１９８７，Ｖｏｌ．５，ｐ．２３７−２５３
Ｊ．Ｓａｍｂｒｏｏｋ他編，”ＭｏｌｅｃｕｌａｒＣｌｏｎｉｎｇ”，Ｎｏ．３，ＳｅｃｏｎｄＥｄｉｔｉｏｎ，ＣｏｌｄＳｐｒｉｎｇＨａｒｂｏｒＬａｂｏｒａｔｏｒｙＰｒｅｓｓ，１９８９，１７．１１−１２

Attorney, Agent or Firm:

Nobuyuki Kaneda (2 others)

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