Title:
【発明の名称】分離用ゲルの製造のための方法およびキット
Document Type and Number:
Japanese Patent JP2003512608
Kind Code:
A
Abstract:
A method for the manufacture of a separation gel, which comprises the steps: (i) providing a polymerization mixture containing a first monomer (I) having a polymerizable unsaturated structure and a second monomer (II) comprising two or more polymerizable unsaturated structures and an initiator, and (ii) polymerizing the mixture. The method is characterized in that monomer (I) is an N-vinyl carboxamide; and that (a) the initiating system for the polymerization comprises a UV photoinitiator and polymerization is initiated by UV irradiating the mixture at a wave-length appropriate for the initiator, and/or (b) preselecting monomer (I) and monomer (II) to have comparable reactivities to become incorporated into the growing polymer. A kit for use in the production of an electrophoretic gel is also described. The kit comprises: (a) a monomer (I) carrying an N-vinyl carboxamide group; (b) a monomer (II) carrying a polymerizable unsaturated structure; and (c) an UV initiator compound, a buffer and/or a denaturing agent; said monomers (I and II) being selected to provide comparable reactivities.
Inventors:
Lena Stig
Ronnie Palmgren
Ronnie Palmgren
Application Number:
JP2001531893A
Publication Date:
April 02, 2003
Filing Date:
October 12, 2000
Export Citation:
Assignee:
Amersham Biosciences Aktiebolag
International Classes:
B01D15/08; B01D57/02; B01J20/281; B01J20/30; B03C5/00; C08F2/48; C08F2/50; C08F26/02; C08F220/20; C08F220/56; C08F226/02; G01N27/447; C08F220/02; (IPC1-7): G01N27/447; B01D15/08; B01D57/02; B01J20/26; B01J20/30; B03C5/00; C08F2/50; C08F220/20; C08F220/56; C08F226/02
Attorney, Agent or Firm:
Aoyama Ryo (2 outside people)