Title:
【発明の名称】移動性調節シアニン色素
Document Type and Number:
Japanese Patent JP2003519275
Kind Code:
A
Abstract:
The present invention provides a novel class of fluorescent cyanine dye compounds that are modified at one of the hetercyclic ring nitrogen atoms with a mobility-modifying moiety that permits the electrophoretic mobilities of polynucleotides labeled with the mobility-modifying cyanine dyes to be adjusted or tuned in a predictable fashion while retaining enzymatic activity. The ability to predictably tune the relative electrophoretic mobilities of the dyes permits the creation of sets of mobility-matched fluorescent dyes of a variety of structures for a variety of applications, including fluorescence-based 4-color nucleic acid sequencing reactions.
Inventors:
Menken, Stephen M.
Benson, Scott Sea.
Rosenblum, Burnett Bee.
Khan, Shire H.
Benson, Scott Sea.
Rosenblum, Burnett Bee.
Khan, Shire H.
Application Number:
JP2001550324A
Publication Date:
June 17, 2003
Filing Date:
January 03, 2001
Export Citation:
Assignee:
Appraera Corporation
International Classes:
C09B23/00; C07F9/24; C07H21/00; C09B1/00; C09B11/28; C09B23/02; G01N27/447; G01N33/52; G01N33/533; (IPC1-7): C09B23/00; C09B11/28
Domestic Patent References:
| JPH05307233A | 1993-11-19 |
Foreign References:
| WO1999005221A1 | 1999-02-04 | |||
| WO1997013810A1 | 1997-04-17 | |||
| WO1997040104A1 | 1997-10-30 |
Attorney, Agent or Firm:
Shusaku Yamamoto (2 outside)