Title:
【発明の名称】新規なDNAチップ
Document Type and Number:
Japanese Patent JP2003527846
Kind Code:
A
Abstract:
The invention concerns a DNA chip system for detecting mutation in a target nucleic acid such that only the DNA comprising the mutation remains on the chip at the end of the process. The invention concerns a method which consists in adding a complementary alphaS-phosphothioatedesoxynucleotide of the mutation is added by means of DNA polymorase at the 3' end of the probe hybridised with the target nucleic acid and in adding an exonuclease so that only the elongated probes are not degraded. The detection of the presence or absence of mutation is carried out by directly or indirectly measuring the presence or the absence of DNA in a specific site on the chip. Advantageously, the chip comprises ISFET transistors or piezoelectric transducers.
Inventors:
Caille, Fabrice
Application Number:
JP2001563632A
Publication Date:
September 24, 2003
Filing Date:
March 01, 2001
Export Citation:
Assignee:
Nucleika
International Classes:
G01N21/64; C12M1/00; C12N15/09; C12Q1/68; C12Q1/6827; C12Q1/6837; G01N27/04; G01N27/06; G01N27/327; G01N27/414; G01N27/416; G01N33/53; G01N33/566; G01N37/00; (IPC1-7): C12N15/09; C12M1/00; C12Q1/68; G01N21/64; G01N27/04; G01N27/06; G01N27/327; G01N27/414; G01N27/416; G01N33/53; G01N33/566; G01N37/00
Attorney, Agent or Firm:
Keiichi Ota