Title:
【発明の名称】血漿分画精製法
Document Type and Number:
Japanese Patent JP2716871
Kind Code:
B2
Abstract:
The present invention describes a process for activating Factor II to Factor IIa by incubating Factor II in the presence of Factor V, Factor Xa, phospholipids, and calcium ions. Each of the factors is prepared from a single impure protein fraction which includes Factors II, V and X. The Factor II, V and X purification procedure comprises the steps of DEAE ligand chromatography and precipitation by the addition of barium chloride. Factor V is recovered from the barium chloride supernatant, and Factors II and X are contained in the barium chloride precipitate. The barium chloride precipitate is dissolved in an aqueous solution and is applied to a chromatographic resin coupled with a ligand which binds Factor X and Factor II weakly or not at all. Factor II is recovered from the fraction, which remains unbound or weakly bound to the Factor X binding ligand. Factor Xa is prepared by recovering Factor X from the ligand during the Factor II preparation procedure and activating the Factor X to Factor Xa by specific proteolytic cleavage.
Inventors:
Harling, Stephen W
Uemura, Hahiro
Noda Munehiro
Shitani, Kenneth Tea.
Uemura, Hahiro
Noda Munehiro
Shitani, Kenneth Tea.
Application Number:
JP50358194A
Publication Date:
February 18, 1998
Filing Date:
July 13, 1993
Export Citation:
Assignee:
Alpha Therapeutics Corporation
International Classes:
A61K38/43; C07K1/18; C07K1/22; C07K1/30; C07K14/435; C07K14/745; C12N9/64; C12N9/74; (IPC1-7): C07K14/745; C07K1/18; C07K1/22; C07K1/30
Domestic Patent References:
| JP1294697A | ||||
| JP59500673A |
Other References:
【文献】米国特許4725673(US,A)
【文献】Chemical Abstracts,vol.71(1969)p.164 要約番号1765f
【文献】Chemical Abstracts,vol.71(1969)p.164 要約番号1765f
Attorney, Agent or Firm:
Takashi Ishida (3 others)