Title:
【発明の名称】組換えオンコナーゼと、その化学的接合体および融合タンパク質
Document Type and Number:
Japanese Patent JP2002512774
Kind Code:
A
Abstract:
Recombinantly-produced Onconase molecules and fusion proteins containing the same are disclosed. The recombinantly-produced Onconase molecule has the sequence of native Onconase, retains the proper folding of native Onconase and has cytotoxic activity similar to that of Onconase purified from oocytes of Rana pipiens. cDNA coding for Onconase is extended by one triplet which codes for N-formyl-methionine. When expressed recombinantly, the mutant Onconase has N-formyl-methionine as the N-terminal amino acid, and glutaminyl as the penultimate N-terminal residue. Following expression, the N-formyl methionine residue is cleaved and the penultimate glutaminyl residues are cyclized to produce Onconase with an N-terminal pyroglutamate residue, and hence the same structure and function as native Onconase.
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Inventors:
Goldenberg, David M
Hansen, Hans
Lun, Shui-On
Hansen, Hans
Lun, Shui-On
Application Number:
JP2000535756A
Publication Date:
May 08, 2002
Filing Date:
March 11, 1999
Export Citation:
Assignee:
Immunomedics, Inc.
International Classes:
A61K38/51; A61P35/00; C07K16/46; C07K19/00; C12N9/22; C12N15/09; C12N15/55; A61K38/00; (IPC1-7): C12N15/09; A61K38/51; A61P35/00; C07K16/46; C07K19/00; C12N9/22
Attorney, Agent or Firm:
Okuyama Shoichi (2 outside)