PURPOSE: To obtain the subject compound in high recovery rate by electrodialytically taking out 2-keto-L-gulonic acid produced using microbes from the fermentation liquor or culture fluid to shorten fermentation time.
CONSTITUTION: In producing 2-keto-L-gulonic acid (2KGA) by fermentation process or microbial reaction process using microbes having ability to produce 2KGA, the 2KGA accumulated in the fermentation liquor or microbial reaction liquor is electrodialytically taken out either singly or in combination with low- molecular cation (e.g. Na+, K+) as counterion. Specifically, for example, a culture fluid from a culture tank or reaction vessel is, as necessary, freed from microbes and introduced into an electrodialytic tank equipped with ion exchange membrane followed by current injection to conduct electrodialysis and separate 2KGA from the culture fluid and concentrate it and then is recovered. The 2KGA-productive microbes is pref. Pseudogluconobacter bacteria such as Pseudogluconobacter saccharoketogenes K591 (FERM BP-1130).
YONETO KENKICHI
YAMAGUCHI TAKAMASA
