Title:
【発明の名称】形質転換株の選択マーカー系
Document Type and Number:
Japanese Patent JPH05502592
Kind Code:
A
Abstract:
A transformant selection system has been developed, particularly for a beta -lactam producing strain, more particularly for P. chrysogenum, by the complementation of a mutation of said beta -lactam producing strain by a homologous selection marker without interfering with beta -lactam biosynthesis. Particularly, in applying said transformant selection system a positive selection agent, for instance fluoroacetate is used for the isolation of fac mutants of said strain, particularly of said beta -lactam producing strain. Furthermore, a gene entitled facA has been isolated from P. chrysogenum.
Inventors:
Van haltingswerth willem
Juan Holcombe Robert Francis Cus Maria
Hauka Robertus Johannes
Bowvenberg Rulov Ali Lance
Juan Holcombe Robert Francis Cus Maria
Hauka Robertus Johannes
Bowvenberg Rulov Ali Lance
Application Number:
JP51746091A
Publication Date:
May 13, 1993
Filing Date:
October 15, 1991
Export Citation:
Assignee:
Gist Brocades Namrose Fennaught Shap
International Classes:
C12N1/15; C12N9/00; C12N15/09; C12N15/80; C12P37/00; C12R1/82; (IPC1-7): C12N1/15; C12N15/52; C12N15/80; C12P37/00
Attorney, Agent or Firm:
Minoru Nakamura (6 outside)