Title:
【発明の名称】組み換えバチルス・スリンギエンシス菌株の発生のためのシャトルベクター
Document Type and Number:
Japanese Patent JP2916255
Kind Code:
B2
Abstract:
A plasmid shuttle vector, bifunctional in Bacillus thuringiensis Bt and E.coli hosts, containing a Bt-derived origin of replication, an origin of replication functional in E.coli, a multiple cloning site, and optionally antibiotic resistance marker genes. The elements of the plasmid shuttle vector are located on removable DNA fragments, making the vector useful for the development of recombinant Bt strains that contain not only one or more insecticidal Bt toxin genes but are also essentially free of DNA from non-Bacillus biological sources.
Inventors:
BAUMU JEIMUZU EI
Application Number:
JP50967391A
Publication Date:
July 05, 1999
Filing Date:
May 14, 1991
Export Citation:
Assignee:
EKOJEN INC
International Classes:
C07K14/325; C12N15/09; C12N15/32; C12N15/75; C12P21/02; C12N1/21; C12R1/07; (IPC1-7): C12N15/09; C12N1/21; C12P21/02
Domestic Patent References:
| JP2119780A |
Other References:
Ａｐｐｌ．Ｅｎｖｉｒｏｎ．Ｍｉｃｒｏｂｉｏｌ．，５３（２）（１９８７），ｐ．４１６−４２１
Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．，２６３（１９８８）ｐ．５６１−５６２
Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．，２６３（１９８８）ｐ．５６１−５６２
Attorney, Agent or Firm:
Heiyoshi Odashima