Title:
【発明の名称】シングルステップ切出し手段
Document Type and Number:
Japanese Patent JP2000507446
Kind Code:
A
Abstract:
The present invention is directed to the genetic transformation using multiple genetic sequences, wherein one of said genetic sequences encodes a polypeptide possessing excision activity, specifically a site-specific recombinase activity linked to a transgene unit and the use of this genetic construct in the removal of transgenes therefrom. The present invention provides the means to produce genetically-transformed organisms, in particular plants, in which selectable marker genes have been removed, thereby facilitating multiple sequential genetic transformation events using the same selectable marker gene. Accordingly, the invention provides the means for regulating transgene expression in genetically-manipulated organisms, for example to promote differentiation, de-differentiation, or any unidirectional developmental shift of a target cell which requires the time-specific expression of a particular gene. The invention is particularly suited to the promotion of specific organogeneses in plants using organogenesis-promoting transgenes, wherein the organs which subsequently develop in said plants are genetically transformed with a desired gene but lack organogenesis-promoting transgenes.
Inventors:
Surin, Brian Peter
Defaiter, Robert Charles
Graham, Michael Wayne
Waterhouse, Peter Michael
Keith, Paul Conrad
Ali, Sharjah
Defaiter, Robert Charles
Graham, Michael Wayne
Waterhouse, Peter Michael
Keith, Paul Conrad
Ali, Sharjah
Application Number:
JP53474397A
Publication Date:
June 20, 2000
Filing Date:
March 27, 1997
Export Citation:
Assignee:
Commonwealth Scientific and Industrial Research Organization
The Australian National University
The Australian National University
International Classes:
A01H5/00; C12N5/10; C12N15/82; C12N15/09; (IPC1-7): C12N15/09; A01H5/00; C12N5/10
Attorney, Agent or Firm:
Takashi Ishida (4 others)